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HeLa cells
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Multiphoton fluorescence image of cultured HeLa cells with a fluorescent protein targeted to the Golgi apparatus (orange), microtubules (green) and counterstained for DNA (cyan). National Center for Microscopy and Imaging Research (NCMIR) View MediaRNase A (1)
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A crystal of RNase A protein created for X-ray crystallography, which can reveal detailed, three-dimensional protein structures. Alex McPherson, University of California, Irvine View MediaYeast cells responding to a glucose shortage
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These yeast cells were exposed to a glucose (sugar) shortage. Mike Henne, University of Texas Southwestern Medical Center. View MediaX-ray co-crystal structure of Src kinase bound to a DNA-templated macrocycle inhibitor 5
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X-ray co-crystal structure of Src kinase bound to a DNA-templated macrocycle inhibitor. Markus A. Seeliger, Stony Brook University Medical School and David R. Liu, Harvard University View MediaCone snail shell
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A shell from the venomous cone snail Conus omaria, which lives in the Pacific and Indian oceans and eats other snails. Kerry Matz, University of Utah View MediaCluster analysis of mysterious protein
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Researchers use cluster analysis to study protein shape and function. Each green circle represents one potential shape of the protein mitoNEET. Patricia Jennings and Elizabeth Baxter, University of California, San Diego View MediaYeast cells with accumulated cell wall material
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Yeast cells that abnormally accumulate cell wall material (blue) at their ends and, when preparing to divide, in their middles. This image was captured using wide-field microscopy with deconvolution. Alaina Willet, Kathy Gould’s lab, Vanderbilt University. View MediaActivated mast cell surface
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A scanning electron microscope image of an activated mast cell. This image illustrates the interesting topography of the cell membrane, which is populated with receptors. Bridget Wilson, University of New Mexico View MediaTiny points of light in a quantum dot
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This fingertip-shaped group of lights is a microscopic crystal called a quantum dot. About 10,000 times thinner than a sheet of paper, the dot radiates brilliant colors under ultraviolet light. Sandra Rosenthal and James McBride, Vanderbilt University, and Stephen Pennycook, Oak Ridge National Laboratory View MediaBacterial symbionts colonizing the crypts of a juvenile Hawaiian bobtail squid light organ
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A light organ (~0.5 mm across) of a Hawaiian bobtail squid, Euprymna scolopes, stained blue. Margaret J. McFall-Ngai, Carnegie Institution for Science/California Institute of Technology, and Edward G. Ruby, California Institute of Technology. View MediaZinc levels in a plant leaf
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Zinc is required for the function of more than 300 enzymes, including those that help regulate gene expression, in various organisms including humans. Suzana Car, Dartmouth College View MediaFruit fly nurse cells transporting their contents during egg development
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In many animals, the egg cell develops alongside sister cells. Adam C. Martin, Massachusetts Institute of Technology. View MediaChromium X-ray source
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In the determination of protein structures by X-ray crystallography, this unique soft (l = 2.29Å) X-ray source is used to collect anomalous scattering data from protein crystals containing light atoms The Southeast Collaboratory for Structural Genomics View MediaBacterial glucose isomerase
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A crystal of bacterial glucose isomerase protein created for X-ray crystallography, which can reveal detailed, three-dimensional protein structures. Alex McPherson, University of California, Irvine View MediaMicroscopy image of bird-and-flower DNA origami
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An atomic force microscopy image shows DNA folded into an intricate, computer-designed structure. Hao Yan, Arizona State University View MediaX-ray co-crystal structure of Src kinase bound to a DNA-templated macrocycle inhibitor 2
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X-ray co-crystal structure of Src kinase bound to a DNA-templated macrocycle inhibitor. Markus A. Seeliger, Stony Brook University Medical School and David R. Liu, Harvard University View MediaCRISPR
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RNA incorporated into the CRISPR surveillance complex is positioned to scan across foreign DNA. Cryo-EM density from a 3Å reconstruction is shown as a yellow mesh. NRAMM National Resource for Automated Molecular Microscopy http://nramm.nysbc.org/nramm-images/ Source: Bridget Carragher View MediaNeutrophil-like cells migrating in a microfluidic chip
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Neutrophil-like cells (blue) in a microfluidic chip preferentially migrating toward LTB4 over fMLP. Caroline Jones, University of Texas at Dallas. View MediaC. elegans trapped by carnivorous fungus
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Real-time footage of Caenorhabditis elegans, a tiny roundworm, trapped by a carnivorous fungus, Arthrobotrys dactyloides. Michael Shribak, Marine Biological Laboratory/University of Chicago. View MediaAxolotls showing nervous system components
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Axolotls—a type of salamander—that have been genetically modified so that various parts of their nervous systems glow purple and green. Prayag Murawala, MDI Biological Laboratory and Hannover Medical School. View MediaHuman skeletal muscle
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Cross section of human skeletal muscle. Image taken with a confocal fluorescent light microscope. Tom Deerinck, National Center for Microscopy and Imaging Research (NCMIR) View MediaIntroduction to Genome Editing Using CRISPR/Cas9
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Genome editing using CRISPR/Cas9 is a rapidly expanding field of scientific research with emerging applications in disease treatment, medical therapeutics and bioenergy, just to name a few. Janet Iwasa View MediaCytoscape network wiring diagram 2
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This image integrates the thousands of known molecular and genetic interactions happening inside our bodies using a computer program called Cytoscape. Trey Ideker, University of California, San Diego View MediaProtein folding video
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Proteins are long chains of amino acids. Each protein has a unique amino acid sequence. It is still a mystery how a protein folds into the proper shape based on its sequence. Theoretical and Computational Biophysics Group View MediaFinding one bug
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A nanometer-sized biosensor can detect a single deadly bacterium in tainted ground beef. How? Weihong Tan, University of Florida in Gainesville View MediaZebrafish head vasculature video
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Various views of a zebrafish head with blood vessels shown in purple. Prayag Murawala, MDI Biological Laboratory and Hannover Medical School. View MediaCell-like compartments emerging from scrambled frog eggs 4
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Cell-like compartments that spontaneously emerged from scrambled frog eggs, with nuclei (blue) from frog sperm. Endoplasmic reticulum (red) and microtubules (green) are also visible. Xianrui Cheng, Stanford University School of Medicine. View MediaColor coding of the Drosophila brain - image
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This image results from a research project to visualize which regions of the adult fruit fly (Drosophila) brain derive from each neural stem cell. Yong Wan from Charles Hansen’s lab, University of Utah. Data preparation and visualization by Masayoshi Ito in the lab of Kei Ito, University of Tokyo. View MediaColor coding of the Drosophila brain - black background
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This image results from a research project to visualize which regions of the adult fruit fly (Drosophila) brain derive from each neural stem cell. Yong Wan from Charles Hansen’s lab, University of Utah. Data preparation and visualization by Masayoshi Ito in the lab of Kei Ito, University of Tokyo. View MediaZebrafish embryo showing vasculature
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A zebrafish embryo. The blue areas are cell bodies, the green lines are blood vessels, and the red glow is blood. Kevin Eliceiri, University of Wisconsin-Madison. View MediaCytonemes in developing fruit fly cells
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Scientists have long known that multicellular organisms use biological molecules produced by one cell and sensed by another to transmit messages that, for instance, guide proper development of organs Sougata Roy, University of California, San Francisco View MediaBacterial cells aggregated above a light-organ pore of the Hawaiian bobtail squid
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The beating of cilia on the outside of the Hawaiian bobtail squid’s light organ concentrates Vibrio fischeri cells (green) present in the seawater into aggregates near the pore-containing tis Margaret J. McFall-Ngai, Carnegie Institution for Science/California Institute of Technology, and Edward G. Ruby, California Institute of Technology. View MediaComputer algorithm
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This computer algorithm plots all feasible small carbon-based molecules as though they were cities on a map and identifies huge, unexplored spaces that may help fuel research into new drug therapies. Aaron Virshup, Julia Contreras-Garcia, Peter Wipf, Weitao Yang and David Beratan, University of Pittsburgh Center for Chemical Methodologies and Library Development View MediaX-ray diffraction pattern from a crystallized cefotaxime-CCD-1 complex
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CCD-1 is an enzyme produced by the bacterium Clostridioides difficile that helps it resist antibiotics. Keith Hodgson, Stanford University. View MediaGenetic mosaicism in fruit flies
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Fat tissue from the abdomen of a genetically mosaic adult fruit fly. Genetic mosaicism means that the fly has cells with different genotypes even though it formed from a single zygote. Akhila Rajan, Fred Hutchinson Cancer Center View MediaEndoplasmic reticulum abnormalities
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Human cells with the gene that codes for the protein FIT2 deleted. Green indicates an endoplasmic reticulum (ER) resident protein. Michel Becuwe, Harvard University. View MediaRat Hippocampus
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This image of the hippocampus was taken with an ultra-widefield high-speed multiphoton laser microscope. Tom Deerinck, NCMIR View MediaProtein purification facility
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The Center for Eukaryotic Structural Genomics protein purification facility is responsible for purifying all recombinant proteins produced by the center. Center for Eukaryotic Structural Genomics View MediaCryo-ET cell cross-section visualizing insulin vesicles
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On the left, a cross-section slice of a rat pancreas cell captured using cryo-electron tomography (cryo-ET). On the right, a color-coded, 3D version of the image highlighting cell structures. Xianjun Zhang, University of Southern California. View MediaScientists display X-ray diffraction pattern obtained with split X-ray beamline
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Scientists from Argonne National Laboratory's Advanced Photon Source (APS) display the first X-ray diffraction pattern obtained from a protein crystal using a split X-ray beam, the first of its kind a GM/CA Collaborative Access Team View MediaMouse Brain Cross Section
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The brain sections are treated with fluorescent antibodies specific to a particular protein and visualized using serial electron microscopy (SEM). Anton Maximov, The Scripps Research Institute, La Jolla, CA View MediaCCP enzyme
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The enzyme CCP is found in the mitochondria of baker’s yeast. Scientists study the chemical reactions that CCP triggers, which involve a water molecule, iron, and oxygen. Protein Data Bank. View MediaA molecular interaction network in yeast 1
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The image visualizes a part of the yeast molecular interaction network. Keiichiro Ono, UCSD View MediaDense tubular matrices in the peripheral endoplasmic reticulum (ER) 1
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Superresolution microscopy work on endoplasmic reticulum (ER) in the peripheral areas of the cell showing details of the structure and arrangement in a complex web of tubes. Jennifer Lippincott-Schwartz, Howard Hughes Medical Institute Janelia Research Campus, Virginia View MediaMapping disease spread
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How far and fast an infectious disease spreads across a community depends on many factors, including transportation. These U.S. David Chrest, RTI International View MediaCRISPR Illustration Frame 5
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This illustration shows, in simplified terms, how the CRISPR-Cas9 system can be used as a gene-editing tool. This is the fifthframe in a series of five. View MediaNano-rainbow
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These vials may look like they're filled with colored water, but they really contain nanocrystals reflecting different colors under ultraviolet light. Shuming Nie, Emory University View MediaBioluminescent imaging in adult zebrafish - overhead view
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Luciferase-based imaging enables visualization and quantification of internal organs and transplanted cells in live adult zebrafish. Kenneth Poss, Duke University View MediaFruit fly ovaries
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Fruit fly (Drosophila melanogaster) ovaries with DNA shown in magenta and actin filaments shown in light blue. This image was captured using a confocal laser scanning microscope.Vladimir I. Gelfand, Feinberg School of Medicine, Northwestern University. View Media
HeLa cells
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Scanning electron micrograph of just-divided HeLa cells. Zeiss Merlin HR-SEM. National Center for Microscopy and Imaging Research View Media